2019-12-25RELRCSBRCSBRCSB2018-09-032018-09-122019-01-162019-12-25National Science Foundation (NSF, United States)DMR-1344290United StatesNational Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)R01HL115153United StatesDepartment of Energy (DOE, United States)DE-AC02-05CH11231United StatesNational Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM104427United StatesSingle-Molecule 3D Image of Human Plasma Intermediate-Density Lipoprotein (No. 09)Lei DYu YKuang YKrauss RRen GLei DYu YKuang YLLiu JKrauss RMRen GSingle-molecule 3D imaging of human plasma intermediate-density lipoproteins reveals a polyhedral structure.Biochim Biophys Acta Mol Cell Biol Lipids1864260270201830557627doi:10.1016/j.bbalip.2018.12.0041879-2618EMD-9077associated EM volumeHuman plasma intermediate-density lipoproteinHuman plasma intermediate-density lipoprotein0Homo sapiensBlood3tomographyparticle27Dulbecco's phosphate buffered saline (DPBS)HomemadeCOPPER200CARBONLACEYGLOW DISCHARGEETHANE80LEICA EM GPHuman IDLs were isolated from the plasma of a healthy individual by non-equilibrium density gradient ultracentrifugationNO SECTIONINGZEISS LIBRA120PLUSFLOOD BEAMBRIGHT FIELDLAB61202.250000.0In-column Omega Filter20GATAN ULTRASCAN 4000 (4k x 4k)409640961.361X-ray speckles in images were removed before CTF correction.FOURIER SPACE82.9FSC 0.5 CUT-OFFThe 3D reconstruction was performed by using individual-particle electron tomography (IPET). The obtained 3D map was low-pass filtered to 8 nm.35