2019-12-25RELRCSBRCSBRCSB2018-09-032018-09-262019-01-022019-12-25National Science Foundation (NSF, United States)DMR-1344290United StatesNational Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)R01HL115153United StatesDepartment of Energy (DOE, United States)DE-AC02-05CH11231United StatesNational Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM104427United StatesSingle-Molecule 3D Image of Monoclonal Antibody MAB012 (No. 05)Lei DYu YKuang YKrauss RRen GLei DYu YKuang YLLiu JKrauss RMRen GSingle-molecule 3D imaging of human plasma intermediate-density lipoproteins reveals a polyhedral structure.Biochim Biophys Acta Mol Cell Biol Lipids1864260270201830557627doi:10.1016/j.bbalip.2018.12.0041879-2618EMD-9099associated EM volumeMonoclonal antibody mAB012Monoclonal antibody mAB0120Mouse monoclonal antibody mAB012, whose epitope located within the first 20 N-terminal residues of human apoB-100, was purchased from EMD Millipore.Mus musculusascitesMus musculustomographyparticle7Dulbecco's phosphate buffered saline (DPBS)HomemadeCOPPER200CARBONLACEYGLOW DISCHARGEAIRETHANE80LEICA EM GPMouse monoclonal antibody mAB012, whose epitope located within the first 20 N-terminal residues of human apoB-100, was purchased from EMD Millipore.NO SECTIONINGFEI TECNAI F20FLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN20019000.0GATAN K2 SUMMIT (4k x 4k)1.111X-ray speckles in images were removed before CTF correction.FOURIER SPACE58.1FSC 0.5 CUT-OFFThe 3D reconstruction was performed by using individual-particle electron tomography (IPET). The obtained 3D map was low-pass filtered to 8 nm.54