2020-07-29RELRCSBRCSBRCSB2018-09-302018-10-242018-11-282020-07-29National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS)R01NS038631United StatesNational Institutes of Health/National Institute of Mental Health (NIH/NIMH)1F32MH115595United StatesDiheteromeric NMDA receptor GluN1/GluN2A in the '2-Knuckle-Asymmetric' conformation, in complex with glycine and glutamate, in the presence of 1 micromolar zinc chloride, and at pH 7.4Jalali-Yazdi FChowdhury SYoshioka CGouaux EJalali-Yazdi FChowdhury SYoshioka CGouaux EMechanisms for Zinc and Proton Inhibition of the GluN1/GluN2A NMDA Receptor.Cell17515201532.e15201830500536doi:10.1016/j.cell.2018.10.0431097-4172EMD-9155associated EM volumeEMD-9147other EM volumeEMD-9148other EM volumeEMD-9149other EM volumeEMD-9150other EM volumeEMD-9151other EM volumeEMD-9152other EM volumeEMD-9153other EM volumeEMD-9154other EM volumeEMD-9156other EM volumeEMD-9157other EM volumeEMD-9158other EM volumeEMD-9159other EM volumeEMD-9160other EM volumeEMD-9161other EM volumeEMD-9162other EM volumeEMD-9163other EM volumeEMD-9164other EM volumeEMD-9165other EM volume6mmlFULLOVERLAPDiheteromeric NMDA receptor GluN1/GluN2A in the '2-Knuckle-Asymmetric' conformation, in complex with glycine and glutamate, in the presence of 1 micromolar zinc chloride, and at pH 7.4Diheteromeric NMDA receptor GluN1/GluN2A in the '2-Knuckle-Asymmetric' conformation, in complex with glycine and glutamate, in the presence of 1 micromolar zinc chloride, and at pH 7.4012Sample was heterologously expressed in TSA-201 cells, detergent solubilized, and affinity purifiedRattus norvegicusHomo sapiensTSA-2010.5Glutamate receptor ionotropic, NMDA 1Rattus norvegicus0.0941897812Homo sapiensLEVOMSTMHLLTFALLFSCSFARAACDPKIVNIGAVLSTRKHEQMFREAVNQANKRHGSWKIQLNATSVTHKPNAIQMALSVCE
DLISSQVYAILVSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRTVPPYSHQSSVWFEMMRVYNW
NHIILLVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLMEARELEARVIILSASEDDAATVYRAAAMLNM
TGSGYVWLVGEREISGNALRYAPDGIIGLQLINGKNESAHISDAVGVVAQAVHELLEKENITDPPRGCVGNTNIWKTGPL
FKRVLMSSKYADGVTGRVEFNEDGDRKFANYSIMNLQNRKLVQVGIYNGTHVIPNDRKIIWPGGETEKPRGYQMSTRLKI
VTIHQEPFVYVKPTMSDGTCKEEFTVNGDPVKKVICTGPNDTSPGSPRHTVPQCCYGFCIDLLIKLARTMNFTYEVHLVA
DGKFGTQERVNNSNKKEWNGMMGELLSGQADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQS
TLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWFSWGVLLNSGIGEGAPRSFSARILGMVWAGFA
MIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKFIYATVKQSSVDIYFRRQVELSTMYRHMEKHNYESAAEAIQA
VRDNKLHAFIWDSAVLEFEASQKCDLVTTGELFFRSGFGIGMRKDSPWKQNVSLSILKSHENGFMEDLDKTWVRYQECDS
RSNAPATLTFENMAGVFMLVAGGIVAGIFLIFIEIAYKGlutamate receptor ionotropic, NMDA 2ARattus norvegicus0.0937403522Homo sapiensLEVOMGRLGYWTLLVLPALLVWRDPAQNAAAEKGPPALNIAVLLGHSHDVTERELRNLWGPEQATGLPLDVNVVALLMNRTDPK
SLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPILGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLK
IMQDYDWHVFSLVTTIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQLKKIHSSVILLYCSKDEAVLIL
SEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGLISVSYDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASC
YGQAEKPETPLHTLHQFMVNVTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENQTLSLRHAVWPRYKSFSDCE
PDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGMNVKKCCKGFCIDILKKLSRTVKFTYDLYLV
TNGKHGKKVNNVWNGMIGEVVYQRAVMAVGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVM
MFVMLLIVSAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVI
FLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRFGTVPNGSTERNIRNNYPYMHQYMTRFNQRGVEDALVSL
KTGKLDAFIYDAAVLNYKAGRDEGCKLVTIGSGYIFATTGYGIALQKGSPWKRQIDLALLQFVGDGEMEELETLWLTGIC
HNEKNEVMSSQLDIDNMAGVFYMLAAAMALSLITFIW2-acetamido-2-deoxy-beta-D-glucopyranose0.00022120830NAGsingleParticleparticle4.07.4150.0NaClSodium Chloride20.0C8H18N2O4SHEPES1.0C56H92O29Digitonin1.0ZnCl2Zinc ChlorideQuantifoil R1.2/1.3GOLD300CARBONHOLEYGLOW DISCHARGEAIRETHANE100291FEI VITROBOT MARK IVSample was blotted for 3 seconds at blot force 1.. This sample was monodisperseFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.7GATAN K2 BASE (4k x 4k)1165322.055.01GctfCleaned-up particles were used in CryoSPARC for de novo model generation.C1FOURIER SPACE7.14FSC 0.143 CUT-OFFcisTEM52489RANDOM ASSIGNMENTRELIONMAXIMUM LIKELIHOODRELIONRELION4PE5A25-8305TQ0B34-3885I57A3-2885UOWB25-832RIGID BODY FITemd_9155_msk_1.mapemd_9155_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
192
192192
0
00192192192330.24330.24330.2490.090.090.0XYZ-4.1171818.5453610.037101970.469855131.71999991.71999991.7199999Half-maps used for FSC calculationsemd_9155_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
192
192192
0
00192192192330.24330.24330.2490.090.090.0XYZ-4.12487038.6019460.0374692270.46871791.71999991.71999991.7199999Half-maps used for FSC calculations