2020-12-02RELRCSBRCSBRCSB2018-10-102018-10-242018-10-242020-12-02National Institutes of Health/National Institute of General Medical SciencesR01-GM081871United StatesOther privateSimons Foundation / SF349247United StatesNational Institutes of Health/National Institute of General Medical SciencesF32GM128303United StatesNational Institutes of Health/National Institute of General Medical SciencesGM103310United StatesOther governmentNYSTARUnited StatesNational Institutes of Health/Office of the DirectorOD019994United StatesOther privateAgouron Institute / F00316United StatesT20S proteasome using Topaz at threshold t-3 (EMPIAR-10025 reprocessing)Bepler TMorin ABrasch JShapiro LNoble AJBerger BBepler TMorin ANoble AJBrasch JShapiro LBerger BPositive-unlabeled convolutional neural networks for particle picking in cryo-electron micrographs.Res Comput Mol Biol10812245247201829707703EMD-9194associated EM volumeEMD-9211other EM volumeEMD-9210other EM volumeEMD-9209other EM volumeEMD-9208other EM volumeEMD-9207other EM volumeEMD-9206other EM volumeEMD-9202other EM volumeEMD-9201other EM volumeT20S proteasomeT20S proteasome0Thermoplasma acidophilumThermoplasma acidophilumsingleParticleparticle0.217.820 mM Tris, 150 mM NaClunspecifiedETHANEGATAN CRYOPLUNGE 3Blot for 2.5 seconds before plunging.
Vitrification carried out at room temperature.. FEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.70.92.422500.0FEI TITAN KRIOS AUTOGRID HOLDERGATAN K2 SUMMIT (4k x 4k)SUPER-RESOLUTION53.01CryoSparc v0 homogeneous 3D refinement. No particle pre-processing.160658All initial particles were used in the final reconstruction; ie. no particle filtering or classification was performed.Cryosparc v0 ab initioD72.83FSC 0.143 CUT-OFFcryoSPARC0CryoSparc v0 homogeneous 3D refinement.160658OTHERCryosparc v0OTHERCryosparc v0emd_9194_msk_1.mapemd_9194_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
400
400400
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00400400400263.0263.0263.090.090.090.0XYZ-0.124991280.37508520.000746184960.0245923080.65750.65750.6575Cryosparc v0 unsharpened map for Topaz t-3 thresholdemd_9194_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
400
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00400400400263.0263.0263.090.090.090.0XYZ-0.225402890.404829170.00088363750.0348678830.65750.65750.6575Cryosparc v0 halfmap A for Topaz t-3 thresholdemd_9194_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
400
400400
0
00400400400263.0263.0263.090.090.090.0XYZ-0.240244090.40521760.000889441060.0348881450.65750.65750.6575Cryosparc v0 halfmap B for Topaz t-3 threshold