2018-10-24RELRCSBRCSBRCSB2018-10-152018-10-242018-10-242018-10-24Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine using Topaz picks thresholded to t-4 (EMPIAR-10028 reprocessing)Bepler TMorin ABrasch JShapiro LNoble AJBerger BBepler TMorin ANoble AJBrasch JShapiro LBerger BPositive-unlabeled convolutional neural networks for particle picking in cryo-electron micrographs.Res Comput Mol Biol10812245247201829707703Wong WBai XCBrown AFernandez ISHanssen ECondron MTan YHBaum JScheres SHCryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine.ElifeUS3201424913268doi:10.7554/eLife.030802050-084XEMD-9207associated EM volumeEMD-9201other EM volumeEMD-9202other EM volumeEMD-9206other EM volumeEMD-9208other EM volumeEMD-9209other EM volumeEMD-9210other EM volumeEMD-9211other EM volumeEMD-9194other EM volumePlasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetinePlasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine0Topaz t-4 picksPlasmodium falciparumPlasmodium falciparumsingleParticleparticle0.67.420 mM Hepes pH7.4, 40 mM KCH3COO, 10 mM NH4CH3COO, 10 mM Mg(CH3COO)2 and 5 mM 2-mecaptoethanolQuantifoil R2/2CARBONGLOW DISCHARGEhome-made continuous carbon filmETHANEFEI VITROBOT MARK IVBlot 2.5 seconds before plunging.. FEI POLARA 300FLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.00.83.878000.GATAN LIQUID NITROGENFEI FALCON II (4k x 4k)20.01CryoSparc v0 homogeneous 3D refinement. No particle pre-processing.228460All initial particles were used in the final reconstruction; ie. no particle filtering or classification was performed.Cryosparc v0 ab initio1C13.0FSC 0.143 CUT-OFFcryoSPARC0228460OTHERCryosparc v0OTHERCryosparc v0emd_9207_msk_1.mapemd_9207_additional.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
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00360360360482.40002482.40002482.4000290.090.090.0XYZ-0.86963852.62802270.0102447420.187885541.341.341.340.9Cryosparc with Topaz t-4 picks half mapemd_9207_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
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00360360360482.40002482.40002482.4000290.090.090.0XYZ-0.88379082.7010750.0103008970.187780021.341.341.340.9Cryosparc with Topaz t-4 picks half map