2024-03-13RELRCSBRCSBRCSB2018-11-062018-12-052019-03-132024-03-13National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)5 R01 AI114975-05United StatesDepartment of Energy (DOE, United States)DE-FG01-91ER20021United StatesCryo-EM structure of the HO BMC shell: Icosahedral reconstruction (main population)Greber BJSutter Mmicrocompartment, shell, compartmentalization, BMC fold, STRUCTURAL PROTEIN, VIRUS LIKE PARTICLEGreber BJSutter MKerfeld CAThe Plasticity of Molecular Interactions Governs Bacterial Microcompartment Shell Assembly.StructureUK27749763.e4201930833088doi:10.1016/j.str.2019.01.0170969-21262005STRUE6EMD-9310other EM volumeEMD-9311other EM volumeEMD-9307other EM volumeEMD-9308other EM volumeEMD-9312other EM volumeEMD-9296other EM volumeEMD-9314other EM volumeEMD-9313other EM volumeEMD-9315other EM volume6mzxFULLOVERLAPBacterial microcompartment shell from Haliangium ochraceumBacterial microcompartment shell from Haliangium ochraceum0123Haliangium ochraceum6.5Ethanolamine utilization protein EutN/carboxysome structural protein CcmlHaliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2)DSM 14365 / JCM 11303 / SMP-20.0098663189999999981Escherichia coliLEVOMVLGKVVGTVVASRKEPRIEGLSLLLVRACDPDGTPTGGAVVCADAVGAGVGEVVLYASGSSARQTEVTNNRPVDATIMA
IVDLVEMGGDVRFRKDD0LHE5Microcompartments protein HO-5815Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2)DSM 14365 / JCM 11303 / SMP-20.0101267186Escherichia coliLEVOMADALGMIEVRGFVGMVEAADAMVKAAKVELIGYEKTGGGYVTAVVRGDVAAVKAATEAGQRAAERVGEVVAVHVIPRPH
VNVDAALPLGRTPGMDKSAD0LID5Microcompartments protein HO-5816Haliangium ochraceumDSM 14365 / JCM 11303 / SMP-20.0229041369999999982Escherichia coliLEVOMSITLRTYIFLDALQPQLATFIGKTARGFLPVPGQASLWVEIAPGIAINRVTDAALKATKVQPAVQVVERAYGLLEVHHF
DQGEVLAAGSTILDKLEVREEGRLKPQVMTHQIIRAVEAYQTQIINRNSQGMMILPGESLFILETQPAGYAVLAANEAEK
AANVHLVNVTPYGAFGRLYLAGSEAEIDAAAEAAEAAIRSVSGVAQESFRDRD0LID6singleParticleparticle37.420.0Tris-HCl50.0Sodium chloride0.01NP-40 substituteC-flat-1.2/1.3COPPER400CARBONHOLEYCARBONCONTINUOUSPLASMA CLEANINGProtochips C-flat 1.2/1.3 holey carbon grids were coated with a thin carbon film and plasma cleaned using a Gatain Solarus.ETHANE100277FEI VITROBOT MARK IV5-7 sec incubation of the sample on the grid before blotting and plunging. FEI TITANFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN30050.02.71.03.548543.0GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDERNITROGENGATAN K2 SUMMIT (4k x 4k)COUNTING383837101-3019284.525.0928 images retained after inspection for image quality. Frames were aligned and dose weighed using Motioncor2.1318001000 particles were picked manually to generate reference templates for subsequent auto-picking in RELION 1.4.EMD-8747The reference was re-scaled and placed in a 512x512x512 voxel box to match the pixel size and appropriate box size for the data.1IFOURIER SPACE3.0FSC 0.143 CUT-OFFFREALIGN9Frequency-limited refinement in FREALIGN, excluding spatial frequencies higher than 3.6 Angstroms to avoid over-fitting. Icosahedral symmetry was applied throughout the refinement procedure.15779MAXIMUM LIKELIHOODRELION1.4PROJECTION MATCHINGFREALIGN9Final refinement in FREALIGN, using defocus refinement and Ewald sphere correction.47950.0RELION1.4Icosahedral symmetry was applied during the classification.5V74PDBexperimental modelOTHERREALemd_9309_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
512
512512
0
00512512512527.36527.36527.3690.090.090.0XYZ-0.03857960.125369460.00196733230.0093938151.031.031.03Unfiltered half-mapemd_9309_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)