2022-03-23RELPDBjPDBjPDBj2016-10-282016-11-022016-12-212022-03-23National Science Foundation of China31270765ChinaThe Key Research and Development Program of MOST2016YFA0501101ChinaThe Beijing Municipal Science & Technology CommissionZ161100000116034ChinaNational Institutes of Health/National Cancer Institute (NIH/NCI)CA168635United StatesNational Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)ES007061United StatesNational Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)ES015252United StatesHuman RAD51 post-synaptic complexesXu JZhao LXu YZhao WSung PWang HWXu JZhao LXu YZhao WSung PWang HWCryo-EM structures of human RAD51 recombinase filaments during catalysis of DNA-strand exchangeNat. Struct. Mol. Biol.US244046201727941862doi:10.1038/nsmb.33361545-9985EMD-9567associated EM volumeHuman RAD51 post-synaptic complexesEMD-9568other EM volumeEMD-9566other EM volume5h1cFULLOVERLAPHuman RAD51 post-synaptic complexHuman RAD51 post-synaptic complex012324.18Human RAD5111DNA12DNA13DNA repair protein RAD51 homolog 1Homo sapiens0.0370080743Escherichia coli 'BL21-Gold(DE3)pLysS AG'LEVOMAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAEAAK
LVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYI
DTEGTFRPERLLAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSA
RQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICQIYDSPCL
PEAEAMFAINADGVGDAKDDNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*T)-3')Homo sapiens0.0026927781(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)DNADNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*A)-3')Homo sapiens0.00277390399999999981(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)DNAMAGNESIUM ION2.4305e-053MGPHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER0.0005061963ANPhelicalfilament0.0757.525mM Tris-HCl, pH 7.5, 50mM KCl, 1mM dithiothreitol, 1mM AMP-PNP and 2mM MgCl2Quantifoil R1.2/1.3COPPER300CARBONHOLEY20.0PLASMA CLEANINGAIR101.325ETHANE100289FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN30070.02.71.51.52.52.5GATAN K2 SUMMIT (4k x 4k)SUPER-RESOLUTION767674205.03-1425288.050.017815.856.18C1BACK PROJECTION4.5FSC 0.143 CUT-OFFSPIDER20.02RELION1.2with helix option30194CTFFIND341146Randomly assigned euler angle for each segment and using back projection to generate initial model.NOT APPLICABLESPIDER20.02RELION1.2with helix option1SZPE80-395REAL