2019-11-06
REL
PDBj
PDBj
PDBj
2018-11-09
2019-01-16
2019-01-16
2019-11-06
Chinese Academy of Sciences
XDB08010301
China
National Science Foundation (China)
31670750
China
Cryo-EM structure of Csm effector complex
Huo Y
Li T
Wang N
Dong Q
Wang X
Jiang T
Huo Y
Li T
Wang N
Dong Q
Wang X
Jiang T
Cryo-EM structure of Type III-A CRISPR effector complex.
Cell Res.
CN
28
1195
1197
2018
30459428
doi:10.1038/s41422-018-0115-6
1748-7838
EMD-9708
associated EM volume
Cryo-EM structure of Csm effector complex
6iqw
FULLOVERLAP
ToCsm effector complex
ToCsm effector complex
0
1
2
3
4
5
6
Thermococcus onnurineus NA1
Escherichia coli
Csm1
Thermococcus onnurineus (strain NA1)
NA1
0.088131898
1
Escherichia coli
LEVO
MEIDELTALGGLLHDIGKPVQRAGLYSGDHSTQGARFLRDLAENTGRAEYELLSLFSEFHHKGHMKNDELMIRRIKELSP
ERFGLTMEDVLNALWIVYEADNLASGEREEGQPQASRPLYSVFNPGKAYPWAELDFEKELPVPGDVFSIRSQDYRELVKR
LWEELSKAKLRSDRLLPVLEKYLTFVSSVTSEGNIISLYDHMRMTSAIALAMLRAGCTAEDVRSGRCRKEKRFLLIEGDF
SGIQDFIYRVSGKGTLKYLRARSAYLELIGWDVVLEILSRLGLTRANVVFNAGGHFMIIAQNTPDAVKELEEIRAKAVEW
LYREFESDLYLAIEWEPVSGREFGREGGKNLFAEARKRLKHKLTVRKLKRFGEIKGLFEHGHTERLAECPVCGRELPEGK
LEPSASDPETKVCPTCNRLVSLGGNLPKLLGFGRTAKNDAGVLVEGPFSGFVPYLQGGRPVGEQILVKNTLNPGEIPESA
QFVPYFVADYFKKDPKGGVATFEELSMASTGTRRLGVMKGDVDRLGEFFSSMDSPSKLATASRFMDYFFKGYIGAIIEGK
FGYIIGDVPSLRDWPEEPDIVVVYAGGDDFFIVGAWDQIFELAFRVRRAFNAYTGGKLTLSVGLGYFDERTPIYRMADVV
SERLDTAKDEGRNRVFVVGRSRPLDGKHKLSYEWNHYEELWRTYAPRIYAGNGRLKGKLESKKGLLWKLLEIRELYVRDP
NDVRWAYLTAYLLGRHGLSDLFPELVGIDTKAVERKEPQPVYWVDGVLKIVLMAVRR
Csm2
Thermococcus onnurineus (strain NA1)
NA1
0.022410691
1
Escherichia coli
LEVO
MHHHHHHVIFVAYHQKHGGYGRGGYGRQDRPQVDASRLFGESPDVVGIKKMLEGKGKQWEAIQPYFDNVVREAKNFLEWS
PNKRLANAVTVAAYLTSQGLKTNQVRKILDMARTTELKVKRGEGDIKDDLVKMRYLLAYTVGKATGQSKYSLDAFHRILD
PMLEVLMGSPKKENFEKFYDFLQAVVAYHKFFGGGD
Csm3
Thermococcus onnurineus (strain NA1)
NA1
0.032721936
2
Escherichia coli
LEVO
MDRRFYGKIVIKGKIKAVTGLHIGSQRDISEIGGIDNPVIKDPHTGLPYIPGSSLKGRLRSLFEILVNSRLGEWREKYPS
LANYSPGSCRPDNQENCGKFFNRKINRGWIHVCPDYETALACPVCRLFGASGKESNFPSRIIVRDAFLTKEWEEKWRAGE
AITEAKIEVGIDRVTSQANPRTNERVVAGAEFEFEIIYNVENTTHWRDDIKNLLTAMALLEDSYLGGSGSRGYGKVKFIF
DSFEFRPLDYYRTGKDEDIVSIDAREKSVSDILSGFDSLFSEVEGKLEAG
Csm4
Thermococcus onnurineus (strain NA1)
NA1
0.032345061
1
Escherichia coli
LEVO
MPKFIAVKLIPKGPFRDIPRADTLFGAIGNAISAIHGQSAVEELVDAFVGGARISSAFPYSGDTYYLPKPLSVEPALEGI
LTGLDEEERYTTAKRLRKAKYLDLKNFELALRLRPFTIPEEIPYARVDVPRVVLDRVTQDSSIYFWEEIRFREKSGVYFL
YSGPREVFDGYIAPAMRFLGDTGIGGKSTWGAGLFEVEFHEMKIDAPGSEYSVTLSNALPTKTPVLWRLLRKGGWSFGRR
KPRMTFIAEGSIVKNDPGGMERLELGLSHEVYVYGLTFPLGVELPEGLE
Csm5
Thermococcus onnurineus (strain NA1)
NA1
0.045262145
1
Escherichia coli
LEVO
MTERTLKVLSPLHIGTGNELTPVDIYPRENIIHVLDTERLVNDLMNLGVELNEILALLKNPPGDAYIWKGYIEEFHLDPS
DYSIYTLKIHGKIGRKSMQIKEFIKLNGRPYIPGSSLKGAIRTAVLYKALKECGDARAVMRVVSKVNGDVARDIGRSEDV
LDYYMSFLSRARIDRKRADDLLEAIVFGMEPDRRSKIRYEPKRDPMKALIVRDSKPVGRKHLAVYHVEVIGNPQPIPIWV
EAIEPGAATDVEIHVDTEALRLNADYFNGLLWECLKERGEPGEVFEDFLWEAVDEFYTAVMKYETIEVQKFGRYTSQVRS
FYASLEDHSGHVLRLGWGSGWLAMTIGLLLVEKGYKWENVRKKLGLGKKPGGSGFSREFPKTRRLADGMPMGWVVLE
RNA (5'-R(*GP*UP*GP*GP*AP*AP*AP*GP*UP*GP*GP*CP*CP*CP*GP*AP*AP*AP*CP*CP*CP*UP*UP*C)-3')
Thermococcus onnurineus (strain NA1)
NA1
0.007707653
1
GUGGAAAGUGGCCCGAAACCCUUC
ADENOSINE-5'-TRIPHOSPHATE
0.000507181
1
ATP
singleParticle
threeDArray
8.0
ETHANE
FEI TITAN KRIOS
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
300
GATAN K2 SUMMIT (4k x 4k)
1.875
1
C1
3.35
FSC 0.143 CUT-OFF
61924
PROJECTION MATCHING
COMMON LINE