2024-03-27
REL
PDBj
PDBj
PDBj
2019-03-07
2019-05-15
2019-05-15
2024-03-27
National Research Foundation (Korea)
NRF-2016R1A2B3006293
Korea, Republic Of
National Research Foundation (Korea)
NRF-2016K1A1A2912057
Korea, Republic Of
National Research Foundation (Korea)
NRF- 2016H1A2A1908806
Korea, Republic Of
cryo-EM structure of DOT1L bound to H2B ubiquitinated nucleosome
Jang S
Song JJ
histone, nucleosome, methylation, GENE REGULATION
Jang S
Kang C
Yang HS
Jung T
Hebert H
Chung KY
Kim SJ
Hohng S
Song JJ
Structural basis of recognition and destabilization of the histone H2B ubiquitinated nucleosome by the DOT1L histone H3 Lys79 methyltransferase.
Genes Dev.
US
33
620
625
2019
30923167
doi:10.1101/gad.323790.118
0890-9369
2056
GEDEEP
EMD-9843
other EM volume
cryo-EM structure of DOT1L_Nucleosome without ubiquitination
6jma
FULLOVERLAP
DOT1L bound to H2B ubiquitinated nucleosome
DOT1L bound to H2B ubiquitinated nucleosome
0
1
2
3
4
5
6
7
Xenopus laevis
0.29
DNA I&J
synthetic construct
0.035168546999999994
2
(DA)(DG)(DA)(DT)(DT)(DC)(DT)(DA)(DC)(DC)(DA)(DA)(DA)(DA)(DG)(DT)(DG)(DT)(DA)(DT)
(DT)(DT)(DG)(DG)(DA)(DA)(DA)(DC)(DT)(DG)(DC)(DT)(DC)(DC)(DA)(DT)(DC)(DA)(DA)(DA)
(DA)(DG)(DG)(DC)(DA)(DT)(DG)(DT)(DT)(DC)(DA)(DG)(DC)(DT)(DG)(DA)(DA)(DT)(DT)(DC)
(DA)(DG)(DC)(DT)(DG)(DA)(DA)(DC)(DA)(DT)(DG)(DC)(DC)(DT)(DT)(DT)(DT)(DG)(DA)(DT)
(DG)(DG)(DA)(DG)(DC)(DA)(DG)(DT)(DT)(DT)(DC)(DC)(DA)(DA)(DA)(DT)(DA)(DC)(DA)(DC)
(DT)(DT)(DT)(DT)(DG)(DG)(DT)(DA)(DG)(DA)(DA)(DT)(DC)(DT)
DNA
Histone H3.2
Xenopus laevis
0.01148841
2
Escherichia coli BL21
LEVO
PHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRV
TIMPKDIQLARRIRGERA
P84233
Histone H4
Xenopus laevis
0.00999077
2
Escherichia coli BL21
LEVO
KRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGR
TLYGFGG
P62799
Histone H2A
Xenopus laevis
0.012660718999999999
2
Escherichia coli BL21
LEVO
AKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAVRNDEEL
NKLLGRVTIAQGGVLPNIQSVLLPKKTESSKSAKSK
Q6AZJ8
Histone H2B 1.1
Xenopus laevis
0.010478031999999998
2
Escherichia coli BL21
LEVO
TRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAV
SEGTKAVTKYTSAK
P02281
Histone-lysine N-methyltransferase, H3 lysine-79 specific
Homo sapiens
0.037930039
1
Escherichia coli BL21
LEVO
LELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDS
IHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTDDDLFVDLGS
GVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVIFVNNFA
FGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILEN
YFSSLKNP
Q8TEK3
2.1.1.43
Ubiquitin
Homo sapiens
0.008576831
1
Escherichia coli BL21
LEVO
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
P0CG47
S-ADENOSYLMETHIONINE
0.000398437
1
SAM
singleParticle
particle
7.5
ETHANE
FEI TITAN
OTHER
BRIGHT FIELD
FIELD EMISSION GUN
300
GATAN K2 SUMMIT (4k x 4k)
37.28
1
6.8
FSC 0.143 CUT-OFF
122242
MAXIMUM LIKELIHOOD
MAXIMUM LIKELIHOOD
FLEXIBLE FIT