2020-02-12RELPDBjPDBjPDBj2019-04-122019-06-122019-06-122020-02-12Chinese Academy of SciencesXDB08020100ChinaStructure of influenza D virus polymerase bound to cRNA promoter in class 2Peng QPeng RQi JGao GFShi YPeng QLiu YPeng RWang MYang WSong HChen YLiu SHan MZhang XWang PYan JZhang BQi JDeng TGao GFShi YStructural insight into RNA synthesis by influenza D polymerase.Nat MicrobiolUK201931209309doi:10.1038/s41564-019-0487-52058-5276EMD-9888associated EM volumeStructure of influenza D virus polymerase bound to cRNA promoter in class 26kuvFULLOVERLAPInfluenza D virus polymerase bound to cRNA promoter in mode B conformationInfluenza D virus polymerase bound to cRNA promoter in mode B conformation012345Influenza D virusSpodoptera frugiperdaHive Five0.27Polymerase 3Influenza D virus0.0830360861Spodoptera frugiperdaLEVOMSSVIREIAKRFLEQATINIAEEVVREYGDHERTMISVGVHFQACCLISDEYTLEDETTPRYVLLEGLKRQEAISKQNNI
CSTLGLEPLRNLADIFDRKTRRFLEVGITKRESDEYYQEKFNKIGNDMDIHVFTYEGKYFSNNPNGLEDIQKTRIFTFLS
FVSDELRKENMFTEMYVTEEGAPELEMYKSKLFIAMRDESVPLPYINYEHLRTRCETFKRNQAECEAKVADVASRLKIKL
EHLEENKLRPLEIPKEKEAPYTHKFLMKDAWFFAKPHDSERAQPQQILYDFFEAANMGFMTTSPKPIFGKQGLMYHSLWG
QTKRAIKDKRNELEPSEQRDFLCGIGRASKKIQEDKWQESREEEFKQEETKGAAKRGFPTWFNEEWLWAMRDSGDGDNKI
GDWIPMAEMPPCKNEMEDYAKKMCEELESKIQGTNCAREMSKLIHTIGSLHTECRNFPGKVKIVPIYCRGTLRGESTDCL
FGIAIKGKSHLNKDDGMYTVVTFEFSTEEPNPSKHEKYTVFEAGTVPVEAVVLTPKRERVLKEKKLFLYCRTTGMSKLKN
DWFSKCRRCLIPTMETVEQIVLKECALKEENRVSEMLENKRAWIAHENGENLTRLVSTKLKDLCRMLIVTQFYYCIYNDN
QLEGFCNEQKKFLMFLQADKDSKSAFTFNQKGLYEKIEECIVSNPLCIFLADRLNKLFLVAKSNGAKYFERNA-directed RNA polymerase catalytic subunitInfluenza D virus0.0861388441Spodoptera frugiperdaLEVOMEINPYLLMLNNDITSMISLTYPYTGAPPMSHGTSTKYSMETVSRTYSYSRTKKEVPSGIFPIERRKFCNTIEDKENLEK
PNGNVDINFMLSLAEMLEEKMGKGFFKFCANEAEAEILKMHFSKLTEGRQTYDWTSERNMPAATALQLTVDAIQETQGTF
KGTTMVEYCNKILEMMDWPEVKFKKVRMIVQRHWDPKTKKEIKMKSPTLMITKIGREEFIKRICTINTMAKDGERGKYKR
RAIATPGMGIRPFSKIVETLAQKICERLAESGLPVGGNEKKAKLKTTVSSTNSKLQEGQFMVNITGDNSKWNECQQPEAY
LAMLAYITKDSSNLMKDLCSVAPTLFCNKYVKMGQGFRAKNKRKTKEIVIPAKKMKERKELMNAEWRDLFETIEPYMDGE
CCFLGGGMLMGMFNMLSTVFGVMTLNYREEALARRNCYWTGLQSSDDFVLFCISRTWPEMEMTILKFIAVCKLMGINMSL
EKSYGCLPELFEFTSMFFSGDFVSNIALELPAFTTAGMNEGTDFTAAMSVIRTNMINNGLSPGTALMALRICLQEFRATY
RVHPYDSGVKNHRMKIIRKFIETIENKDGLLISDGGKLMNNISSLHIPEEILKEDLMDPSYRNRVFNPRNPFTQFEKTVD
IFKASGPIRVEENEAVVSTHSFRTRSNRTLLNTDMRAMALEEKRYQVVCNMYRSVFESADVNTPIGSMSMGEAIEAKILD
RARTQFENGIIGGEEYSEIKRLIEDAKRQRLSV2.7.7.48Polymerase PB2Influenza D virus0.0884804841Spodoptera frugiperdaLEVOMSLLLTLAKEYANLTKDKKSCKLLSQGTVSSYTTFKKWTTSRKEKNPSLRMRWAMGSKFPIMANREILEEAGIPEQWEGI
DLWSKKDDVSKLGMVLASPAAITYWNFCGPGVDNSSVIKDVYKAKFMKKERWRETLWGPMNFELVGKQRRVVETQPVEIK
LNQKEIKELTMWVLFEDEANLASKFIQENFSLVLSLRELYKGKAVNKDVAAFMIAHQFSPEKRFLPTFGPIRPERMELLH
CLGGDFWKIEAVTAGSLNEEQKKRDVRAVARKICLRASVDLFTPAEKIRDYIASVTMRFGTVERTFEDVIRNSDDISAEV
TLCKAALGCELGKSMSFGNLNLRKVSGEAETMEKTVYWGLKPIKYKCWRGEETFYCELRKVTCMFRRSEGLDWANIGPGS
PEERRELLAMVMIFCRDGRFFESAPVNIDESFFRTRLNKEIPYQYVLLKWVRQSRDNLDALLSTRGLIPAHIGQFGKGMG
IDGSSSSSMVYKGVMLSKTPIDIVESKEKHRLFLNDNIEAVTERGAMVASIMDLSEDNRETFNDVTFNHVDLAVLKDEKT
AIIKIYRSLVERINTDDDGLPALIMGKRYLELYQLDEVKDAVGLIPKRMLGAYSYQARQLIQSQIKNDSYSLPEIIKLLP
FCYSPPKKMLFDGTFHFKNQMYVRPGINTNLFSFSKTDKSKIYVNGSAVKIKLVLGDDEMDTSLAFVEGFQVCEYDPRAP
LIPRRDLRLIGFGKKVRVFVGQGQEKTLVRTSSKRAASHDVSKNIRRMRLEVRNA (5'-R(P*UP*CP*CP*UP*UP*GP*CP*UP*AP*CP*UP*GP*CP*U)-3')synthetic construct0.0043375631UCCUUGCUACUGCURNA (5'-R(P*AP*GP*CP*AP*UP*AP*AP*GP*CP*AP*GP*GP*AP*GP*A)-3')synthetic construct0.0049020421AGCAUAAGCAGGAGAsingleParticleparticle0.87.5ETHANE100277FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGATAN K2 QUANTUM (4k x 4k)SUPER-RESOLUTION40.01CTFFIND4.16AB7Influenza D virus plolymeraseC14.1FSC 0.143 CUT-OFFRELION2.054216MAXIMUM LIKELIHOODRELION2.0MAXIMUM LIKELIHOODRELION2.0RELION2.0FLEXIBLE FITREAL