2021-02-10RELPDBjPDBjPDBj2019-06-072019-08-282019-08-282021-02-10Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1 state class2)Hiraizumi MYamashita KNishizawa TNureki OHiraizumi MYamashita KNishizawa TNureki OCryo-EM structures capture the transport cycle of the P4-ATPase flippase.ScienceUS36511491155201931416931doi:10.1126/science.aay33531095-92030038SCIEASEMD-9932associated EM volumeCryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1 state class2)6k7hFULLOVERLAPATP8A1-CDC50aATP8A1-CDC50a012Homo sapiensHomo sapiensExpi293FpcDNA3.4Phospholipid-transporting ATPaseHomo sapiens0.1305378521Homo sapiensLEVOGSREFMPTMRRTVSEIRSRAEGYEKTDDVSEKTSLADQEEVRTIFINQPQLTKFCNNHVSTAKYNIITFLPRFLYSQFRR
AANSFFLFIALLQQIPDVSPTGRYTTLVPLLFILAVAAIKEIIEDIKRHKADNAVNKKQTQVLRNGAWEIVHWEKVNVGD
IVIIKGKEYIPADTVLLSSSEPQAMCYIETSNLDGETNLKIRQGLPATSDIKDVDSLMRISGRIECESPNRHLYDFVGNI
RLDGHGTVPLGADQILLRGAQLRNTQWVHGIVVYTGHDTKLMQNSTSPPLKLSNVERITNVQILILFCILIAMSLVCSVG
SAIWNRRHSGKDWYLNLNYGGASNFGLNFLTFIILFNNLIPISLLVTLEVVKFTQAYFINWDLDMHYEPTDTAAMARTSN
LNEELGQVKYIFSDKTGTLTCNVMQFKKCTIAGVAYGQNSQFGDEKTFSDSSLLENLQNNHPTAPIICEFLTMMAVCHTA
VPERERDKIIYQAASPDEGALVRAAKQLNFVFTGRTPDSVIIDSLGQEERYELLNVLEFTSARKRMSVIVRTPSGKLRLY
CKGADTVIYDRLAETSKYKEITLKHLEQFATEGLRTLCFAVAEISESDFQEWRAVYQRASTSVQNRLLKLEESYELIEKN
LQLLGATAIEDKLQDQVPETIETLMKADIKIWILTGDKQETAINIGHSCKLLKKNMGMIVINEGSLDGTRETLSRHCTTL
GDALRKENDFALIIDGKTLKYALTFGVRQYFLDLALSCKAVICCRVSPLQKSEVVEMVKKQVKVVTLAIGDGANDVSMIQ
TAHVGVGISGNEGLQAANSSDYSIAQFKYLKNLLMIHGAWNYNRVSKCILYCFYKNIVLYIIEIWFAFVNGFSGQILFER
WCIGLYNVMFTAMPPLTLGIFERSCRKENMLKYPELYKTSQNALDFNTKVFWVHCLNGLFHSVILFWFPLKALQYGTAFG
NGKTSDYLLLGNFVYTFVVITVCLKAGLETSYWTWFSHIAIWGSIALWVVFFGIYSSLWPAIPMAPDMSGEAAMLFSSGV
FWMGLLFIPVASLLLDVVYKVIKRTAFKTLVDEVQELEAKSQDPGAVVLGKSLTERAQLLKNVFKKNHVNLYRSESLQQN
LLHGYAFSQDENGIVSQSEVIRAYDTTKQRPDEW7.6.2.1Cell cycle control protein 50AHomo sapiens0.0407275271Homo sapiensLEVOMAMNYNAKDEVDGGPPCAPGGTAKTRRPDNTAFKQQRLPAWQPILTAGTVLPIFFIIGLIFIPIGIGIFVTSNNIREIEI
DYTGTEPSSPCNKCLSPDVTPCFCTINFTLEKSFEGNVFMYYGLSNFYQNHRRYVKSRDDSQLNGDSSALLNPSKECEPY
RRNEDKPIAPCGAIANSMFNDTLELFLIGNDSYPIPIALKKKGIAWWTDKNVKFRNPPGGDNLEERFKGTTKPVNWLKPV
YMLDSDPDNNGFINEDFIVWMRTAALPTFRKLYRLIERKSDLHPTLPAGRYSLNVTYNYPVHYFDGRKRMILSTISWMGG
KNPFLGIAYIAVGSISFLLGVVLLVINHKYRNSSNTADITI2-acetamido-2-deoxy-beta-D-glucopyranose0.0002212082NAGCHOLESTEROL HEMISUCCINATE0.0004867261Y01singleParticleparticle47.5ETHANEFEI TITAN KRIOSOTHERBRIGHT FIELDFIELD EMISSION GUN300GATAN K3 BIOQUANTUM (6k x 4k)64.013.22FSC 0.143 CUT-OFF424465MAXIMUM LIKELIHOODMAXIMUM LIKELIHOODemd_9932_msk_1.mapemd_9932_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
260
260260
0
00260260260215.8215.8215.890.090.090.0XYZ-0.022059410.046623386-3.1825097e-050.00262633830.830.830.83emd_9932_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)