2024-03-27RELPDBjPDBjPDBj2019-07-202019-09-112019-09-112024-03-27National Science Foundation (China)ChinaCryo-EM structure of human MLL1-ubNCP complex (4.0 angstrom)Huang JXue Hhistone modification, nucleosome, MLL, TRANSCRIPTION, TRANSCRIPTION-DNA complexXue HYao TCao MZhu GLi YYuan GChen YLei MHuang JStructural basis of nucleosome recognition and modification by MLL methyltransferases.NatureUK573445449201931485071doi:10.1038/s41586-019-1528-11476-46870006NATUAS6kivFULLOVERLAPHuman MLL1 complex associated with an H2B-monoubiquitinated nucleosome (4.0 angstrom)Human MLL1 complex associated with an H2B-monoubiquitinated nucleosome (4.0 angstrom)01234567891011Human MLL1 complex11234567891011Homo sapiensH2B-monoubiquitinated nucleosome11234567891011Xenopus laevisHistone H3Xenopus laevis0.015303932Escherichia coliLEVOARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKT
DLRFQSSAVMALQEASEAYLVALFEDTNLCAIHAKRVTIMPKDIQLARRIRGERAA0A310TTQ1Histone H4Xenopus laevis0.0112632309999999992Escherichia coliLEVOSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKT
VTAMDVVYALKRQGRTLYGFGGP62799Histone H2AXenopus laevis0.0139782409999999992Escherichia coliLEVOSGRGKQGGKTRAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIP
RHLQLAVRNDEELNKLLGRVTIAQGGVLPNIQSVLLPKKTESSKSAKSKQ6AZJ8Histone H2B 1.1Xenopus laevis0.0134987152Escherichia coliLEVOAKSAPAPKKGSKKAVTKTQKKDGKKRRKTRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRLAHY
NKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTCYTSAKP02281DNA (145-MER)synthetic construct0.0445213671(DT)(DC)(DG)(DA)(DG)(DA)(DA)(DT)(DC)(DC)(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG)
(DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG)(DA)
(DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG)(DC)(DA)(DC)(DC)(DG)(DC)(DT)(DT)(DA)(DA)(DA)
(DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC)(DC)(DC)(DC)
(DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG)(DG)(DG)
(DG)(DA)(DT)(DT)(DA)(DC)(DT)(DC)(DC)(DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA)(DG)
(DG)(DC)(DA)(DC)(DG)(DT)(DG)(DT)(DC)(DA)(DG)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DC)(DA)
(DT)(DC)(DC)(DG)(DA)DNADNA (145-MER)synthetic construct0.0449926479999999961(DT)(DC)(DG)(DG)(DA)(DT)(DG)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DC)(DT)(DG)(DA)(DC)(DA)
(DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DC)(DT)(DA)(DG)(DG)(DG)(DA)(DG)
(DT)(DA)(DA)(DT)(DC)(DC)(DC)(DC)(DT)(DT)(DG)(DG)(DC)(DG)(DG)(DT)(DT)(DA)(DA)(DA)
(DA)(DC)(DG)(DC)(DG)(DG)(DG)(DG)(DG)(DA)(DC)(DA)(DG)(DC)(DG)(DC)(DG)(DT)(DA)(DC)
(DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA)(DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA)(DG)
(DA)(DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA)(DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)(DA)
(DG)(DC)(DG)(DG)(DC)(DC)(DT)(DC)(DG)(DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DT)
(DC)(DT)(DC)(DG)(DA)DNAHistone-lysine N-methyltransferase 2AHomo sapiens0.0249705391Escherichia coliLEVONEPPLNPHGSARAEVHLRKSAFDMFNFLASKHRQPPEYNPNDEEEEEVQLKSARRATSMDLPMPMRFRHLKKTSKEAVGV
YRSPIHGRGLFCKRNIDAGEMVIEYAGNVIRSIQTDKREKYYDSKGIGCYMFRIDDSEVVDATMHGNAARFINHSCEPNC
YSRVINIDGQKHIVIFAMRKIYRGEELTYDYKFPIEDASNKLPCNCGAKKCRKFLNQ031642.1.1.43Retinoblastoma-binding protein 5Homo sapiens0.0592234771Escherichia coliLEVOMNLELLESFGQNYPEEADGTLDCISMALTCTFNRWGTLLAVGCNDGRIVIWDFLTRGIAKIISAHIHPVCSLCWSRDGHK
LVSASTDNIVSQWDVLSGDCDQRFRFPSPILKVQYHPRDQNKVLVCPMKSAPVMLTLSDSKHVVLPVDDDSDLNVVASFD
RRGEYIYTGNAKGKILVLKTDSQDLVASFRVTTGTSNTTAIKSIEFARKGSCFLINTADRIIRVYDGREILTCGRDGEPE
PMQKLQDLVNRTPWKKCCFSGDGEYIVAGSARQHALYIWEKSIGNLVKILHGTRGELLLDVAWHPVRPIIASISSGVVSI
WAQNQVENWSAFAPDFKELDENVEYEERESEFDIEDEDKSEPEQTGADAAEDEEVDVTSVDPIAAFCSSDEELEDSKALL
YLPIAPEVEDPEENPYGPPPDAVQTSLMDEGASSEKKRQSSADGSQPPKKKPKTTNIELQGVPNDEVHPLLGVKGDGKSK
KKQAGRPKGSKGKEKDSPFKPKLYKGDRGLPLEGSAKGKVQAELSQPLTAGGAISELLQ15291UbiquitinHomo sapiens0.0086229221Escherichia coliLEVOMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGCP62979Set1/Ash2 histone methyltransferase complex subunit ASH2Homo sapiens0.0602887581Escherichia coliLEVOMDTQAGSVDEENGRQLGEVELQCGICTKWFTADTFGIDTSSCLPFMTNYSFHCNVCHHSGNTYFLRKQANLKEMCLSALA
NLTWQSRTQDEHPKTMFSKDKDIIPFIDKYWECMTTRQRPGKMTWPNNIVKTMSKERDVFLVKEHPDPGSKDPEEDYPKF
GLLDQDLSNIGPAYDNQKQSSAVSTSGNLNGGIAAGSSGKGRGAKRKQQDGGTTGTTKKARSDPLFSAQRLPPHGYPLEH
PFNKDGYRYILAEPDPHAPDPEKLELDCWAGKPIPGDLYRACLYERVLLALHDRAPQLKISDDRLTVVGEKGYSMVRASH
GVRKGAWYFEITVDEMPPDTAARLGWSQPLGNLQAPLGYDKFSYSWRSKKGTKFHQSIGKHYSSGYGQGDVLGFYINLPE
DTETAKSLPDTYKDKALIKFKSYLYFEEKDFVDKAEKSLKQTPHSEIIFYKNGVNQGVAYKDIFEGVYFPAISLYKSCTV
SINFGPCFKYPPKDLTYRPMSDMGWGAVVEHTLADVLYHVETEVDGRRSPPWEPQ9UBL3WD repeat-containing protein 5Homo sapiens0.0366354381Escherichia coliLEVOMATEEKKPETEAARAQPTPSSSATQSKPTPVKPNYALKFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFE
KTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKT
GKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKL
WDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGHTDVVISTACHPTENIIASAA
LENDKTIKLWKSDCP61964S-ADENOSYL-L-HOMOCYSTEINE0.0003844111SAHZINC ION6.5409e-051ZNsingleParticleparticle7.5ETHANEFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300FEI FALCON III (4k x 4k)40.01C14.0FSC 0.143 CUT-OFF127898MAXIMUM LIKELIHOODMAXIMUM LIKELIHOODemd_9999_msk_1.mapemd_9999_additional_4.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
270
270270
0
00270270270294.30002294.30002294.3000290.090.090.0XYZ-0.12841880.2062486-0.000246104840.0093063661.091.091.09additional map shows EM densities of ASH2L Pre-SPRY domainemd_9999_additional_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
270
270270
0
00270270270294.30002294.30002294.3000290.090.090.0XYZ-0.142093720.23179442-0.000313732160.010047811.091.091.09additional map shows the minor binding mode 1 of H2BK120ub1 to RBBP5emd_9999_additional_3.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
270
270270
0
00270270270294.30002294.30002294.3000290.090.090.0XYZ-0.121876950.21986315-0.000163460510.0103363231.091.091.09additional map shows the minor binding mode 2 of H2BK120ub1 to RBBP5emd_9999_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
270
270270
0
00270270270294.30002294.30002294.3000290.090.090.0XYZ-0.207883860.3480923-0.000370782250.0115425851.091.091.09additional map shows the major binding mode of H2BK120ub1 to RBBP5